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Brain Death Determination

Educational overview of brain death clinical criteria, prerequisites, examination, apnea testing, and ancillary testing. Based on AAN guidelines. Pending institutional review.

Source
AAN Practice Parameters: Brain Death Determination
Status
review pending
Updated
5/29/2026
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⚠️ Content Status

Guideline-supported example content* — Based on AAN Practice Parameters. This is NOT official local protocol.
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  • Guideline-supported example content* — Based on AAN Practice Parameters. This is NOT official local protocol.
Brain death determination is a formal medical, ethical, and legal process. Always follow your institution's specific brain death protocol. Consult neurology, neurosurgery, and/or intensivist per your institutional policy. Do not substitute this content for institutional protocol.

Definition

Brain death* (brain death/death by neurological criteria — BD/DNC) is the irreversible cessation of all brain function, including...
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  • Brain death* (brain death/death by neurological criteria — BD/DNC) is the irreversible cessation of all brain function, including the brainstem.

Prerequisites (Must Be Established Before Exam)

Known cause of coma — structural or metabolic etiology identified and consistent with brain death
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  1. Known cause of coma — structural or metabolic etiology identified and consistent with brain death
  2. No reversible causes of coma:
  • Significant hypothermia: Core temp must be ≥ 36°C (96.8°F)
  • Metabolic disturbances corrected (severe hypoglycemia, hyperosmolarity)
  • Drugs: No CNS depressants (benzodiazepines, opioids, barbiturates, antiepileptics), no neuromuscular blocking agents, alcohol ≥ 0
  • Sufficient time for drug clearance (depends on drug, dose, duration, organ function)
  1. Hemodynamic stability — adequate perfusion during exam

Clinical Examination for Brain Death

No purposeful motor response to stimuli • No evidence of consciousness • No sleep-wake cycling
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Coma

  • No purposeful motor response to stimuli
  • No evidence of consciousness
  • No sleep-wake cycling

Absence of Brainstem Reflexes

ReflexAbsent response in brain death
PupillaryFixed pupils, no response to bright light (mid-position or fully dilated)
CornealNo blink to corneal touch bilaterally
Oculocephalic (Doll's eyes)No eye movement with rapid head rotation (do NOT test if C-spine not cleared)
Oculovestibular (cold caloric)No eye movement after cold water irrigation bilaterally
GagNo gag response to posterior pharynx stimulation
CoughNo cough to deep tracheal suctioning
  • Note:* Spinal reflexes (including Babinski, triple flexion) may persist and are not evidence of brainstem function.

Apnea Test

Pre-oxygenate: FiO₂ 1.0 for ≥ 10 minutes; verify PaO₂ ≥ 200 mmHg • Disconnect ventilator • Provide diffusion oxygenation (e. g., 6...
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  1. Pre-oxygenate: FiO₂ 1.0 for ≥ 10 minutes; verify PaO₂ ≥ 200 mmHg
  2. Disconnect ventilator
  3. Provide diffusion oxygenation (e. g., 6 L/min O₂ via suction catheter in ET tube)
  4. Observe for spontaneous respiratory effort for 8–10 minutes
  5. Check ABG at end:
  • Positive test (confirms brain death): PaCO₂ ≥ 60 mmHg (or ≥ 20 mmHg above baseline) with no respiratory effort
  • Abort if:* hemodynamic instability, SpO₂ < 85% for > 30 seconds, significant arrhythmia.

Number of Examinations Required

Many institutions require 2 examinations by 2 physicians (typically > 6 hours apart) • At least one examination should typically...
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  • Many institutions require 2 examinations by 2 physicians (typically > 6 hours apart)
  • At least one examination should typically be by an attending with training in brain death determination
  • Follow your institution's specific protocol

Ancillary Testing

Test • What It Shows • EEG
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TestWhat It Shows
EEGElectrocerebral silence
Cerebral angiographyNo intracranial circulation
Nuclear medicine cerebral perfusion scanNo flow to brain
CTA head/neckNo intracranial flow
TCD (transcranial Doppler)No cerebral blood flow

Documentation

Date and time of declaration • Documentation of clinical exam findings • Apnea test results
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  • Date and time of declaration
  • Documentation of clinical exam findings
  • Apnea test results
  • Presence of any ancillary tests
  • Attestation by qualified physician(s) per institutional policy

Organ Donation

Once brain death is declared, contact organ procurement organization (OPO) per institutional protocol. OPO will guide referral...
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Once brain death is declared, contact organ procurement organization (OPO) per institutional protocol. OPO will guide referral process and family communication.

  • This is guideline-supported example educational content based on AAN Practice Parameters. Always follow your institution's brain death protocol. Brain death determination has specific medical, legal, and ethical requirements that are institution-specific.*